Silvia Campello

Honors and Fellowships

FEBS Short Term Fellowship, EMBO Short Term Fellowship, FNS Short Term Fellowship (2010); Marie Curie Intra-European Fellowship – FP7-PEOPLE-IEF-2008 (2009); Roche Research Foundation Fellowship (2008); Collaboration fellowship (“Assegno di Ricerca”), University and Research Italian Ministry (MIUR) (2006-7); Annual fellowship for the participation to the Project C.E.E. Socrates-Erasmus scholarship at the University Paris XI of Orsay, France (1998).

Funding

2009 – Foundation Schmidheiny, Svizzera

2010 – Société Académique de Genève, Svizzera

– Gertrude von Meissner Foundation, Svizzera

2011 – Grant “Giovani Ricercatori” GR-2009-1606827, Bando 2009, Ministero della Salute, Italia

– MyFirst Grant MFAG 12120, AIRC, Italia

2014 – Grant “Giovani Ricercatori” GR-2011-02351643, Bando 2011, Ministero della Salute, Italia

2017 – Grant IG, AIRC, Italia

Professional Memberships

2008-present The European Cell Death Organization;

2007-present The Biochemical Society;

2004-present Italian Society of Clinical Immunology and Allergology;

2000-present Italian Group of Bioenergetics and Biomembranes.

Research field

Interest of our group is to investigate on the role of mitochondrial morphology in immunological processes fundamental for T cell physiology. We found that mitochondrial fission promotes lymphocyte chemotaxis, by unraveling a previously unexpected role for morphological mitochondrial adaptations in the immune system. We are now investigating on the role of mitochondrial dynamics in lymphocyte development and transformation, where migration is decisive. Moreover, the physiological apoptosis called Activation-Induced Cell Death (AICD) is essential in the generation and selection of a functional lymphocyte repertoire to avoid autoimmunity. We are also investigating on the crucial role that the mitochondria morphology has in the amplification of AICD and in autoimmune diseases, such as Multiple Sclerosis. In vitro, as well in vivo approaches are used in our studies, in combination with advanced microscopy technologies. Our group, indeed, developed in recent years an outstanding expertise on studying and analyzing mitochondrial morphology, so “touching” fundamental cell processes like apoptosis and autophagy.

Publications

• C. Rodolfo, S. Campello and F. Cecconi (2017) “Mitophagy in neurodegenerative diseases” Neurochem Int. Aug 8. pii: S0197-0186(17)30087-6. doi: 10.1016/j.neuint.2017.08.004 [Epub ahead of print].

• L. Simula, F. Nazio, S. Campello* (2017) “The mitochondrial dynamics in cancer and immune-surveillance” Semin Cancer Biol. 2017 Jun 24. pii: S1044-579X(17)30163-3. doi: 10.1016/j.semcancer.2017.06.007. [Epub ahead of print].

• M. Bordi, F. Nazio, S. Campello* (2017) “The Close Interconnection between Mitochondrial Dynamics and Mitophagy in Cancer” Front Oncol. 7:81. doi: 10.3389/fonc.2017.00081. eCollection 2017.

• F. Nazio, M. Carinci, C. Valacca, P. Bielli, F. Strappazzon, M. Antonioli, F. Ciccosanti, C. Rodolfo, S. Campello, G.M. Fimia, C. Sette, P. Bonaldo, F. Cecconi (2016) “Fine-tuning of ULK1 mRNA and protein levels is required for autophagy oscillation” J Cell Biol. 215,841-856.

• Corrado M. and Campello S.* (2016) “Autophagy inhibition and mitochondrial remodeling join forces to amplify apoptosis in activation-induced cell death” Autophagy 12, 2496-2497. doi: 10.1080/15548627.2016.1226738.

• M. Corrado, F.R. Mariotti, L. Trapani, L. Taraborrelli, F. Nazio, V. Cianfanelli, M.E. Soriano, E. Schrepfer, F. Cecconi, L. Scorrano and S. Campello*. (2016) “Macroautophagy inhibition maintains fragmented mitochondria to foster T cell receptor-dependent apoptosis” The EMBO journal 35,1793-809. doi: 10.15252/embj.201593727.

• S. Campello and F. Cecconi. (2016) “Fanconi Anemia Genes, of Menders and Sweepers” Dev Cell. 37, 299-300. doi: 10.1016/j.devcel.2016.05.011

• M. Corrado, L. Scorrano, S. Campello*. (2016) “Changing perspective on oncometabolites: from metabolic signature of cancer to tumorigenic and immunosuppressive agents.” Oncotarget. 7, 46692-46706. doi: 10.18632/oncotarget.8727.

• D.J. Klionsky, .., S. Campello, et al. (2016) “Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).” Autophagy. 12,1-222. doi: 10.1080/15548627.2015.1100356.

• G. Di Giacomo, S. Campello, M. Corrado, L. Di Giambattista, C. Cirotti, G. Filomeni, G. Gentile. (2015) “Mature Erythrocytes of Iguana iguana (Squamata, Iguanidae) Possess Functional Mitochondria.” PLoS One. Sep 14;10(9):e0136770. doi: 10.1371/journal.pone.0136770.

• A.Ferreira-da-Silva, C. Valacca, E. Rios, H. Pópulo, P. Soares, M. Sobrinho-Simões, L. Scorrano, V. Máximo, S. Campello. (2015) “Mitochondrial dynamics protein Drp1 is overexpressed in oncocytic thyroid tumors and regulates cancer cell migration.” PLoS One. Mar 30;10(3):e0122308. doi: 10.1371/journal.pone.0122308.

• F.R. Mariotti, M. Corrado and S. Campello [corresponding auth.]. (2015) “Following Mitochondria Dynamism: confocal analysis of the organelle morphology”. In “Mitochondrial Regulation: Methods and Protocols”, C.M. Palmeira; A. Pinto Rolo (Eds.) Methods Mol Biol. 1241, 153-61.

• F. Strappazzon, F. Nazio, M. Corrado, V. Cianfanelli, A. Romagnoli, G.M. Fimia, S. Campello, R. Nardacci, M. Piacentini, M. Campanella, F. Cecconi. (2014) “AMBRA1 is able to induce mitophagy via LC3 binding, regardless of PARKIN and p62/SQSTM1.” Cell Death Differ. doi: 10.1038/cdd.2014.139. [Epub ahead of print]

• A.F. da Silva, F.R. Mariotti, V. Máximo, S. Campello. (2014) “Mitochondria dynamism: of shape, transport and cell migration.” Cell Mol Life Sci. 71, 2313-24.

• S. Campello, F. Cecconi. (2014) “Ho(a)xing Autophagy to Regulate Development.” Dev Cell. 28, 3-4.

• S. Campello [correspondig auth.], F. Strappazzon, F. Cecconi. (2014) “Mitochondrial dismissal in mammals, from protein degradation to mitophagy.” Biochim Biophys Acta. 1837, 451-460.

• S. Caserta, S. Campello, G. Tomaiuolo, L. Sabetta and S. Guido. (2013) “A Methodology to Study Chemotaxis in 3D Collagen Gels”. AIChE Journal. 59, 4025-35. (doi: 10.1002/aic.14164).

• M. Corrado, L. Scorrano and S. Campello. (2012) “Mitochondrial dynamics in cancer and neurodegenerative and neuroinflammatory diseases”. Int. J. Cell. Biol. 2012:729290. doi:  10.1155/2012/729290. Epub 2012 Jun 27.

• F. Strappazzon, S. Campello and F. Cecconi. (2012) “Non-apoptotic roles for death-related molecules: when mitochondria chose cell fate”. Exp. Cell. Res. 318, 1309-15.

• F. Strapazzon, M. Vietri-Rudan, S. Campello, F. Nazio, F. Florenzano, G.M. Fimia, M. Piacentini, B. Levine and F. Cecconi. (2011) “Mitochondrial BCL-2 inhibits AMBRA1-induced autophagy”. EMBO j. 30, 1195-1208.

• R.L. Contento, S. Campello, A.E. Trovato, E. Magrini and A. Viola. (2010) “Adhesion shapes T cells for prompt and sustained T cell receptor signaling”. EMBO j. 29, 4035-47.

• S. Campello and L. Scorrano. (2010) “Mitochondrial shape changes: orchestrating cell patho-physiology”. EMBO Reports. 11, 678-84.

• S. Campello, M. Beltramini, G. Giordano, P. Di Muro, S. Marino and L. Bubacco; (2007) “Role of the tertiary structure in the diphenol oxidase activity of Octopus vulgaris hemocyanin”. Arch. Biochem. Biophys. 471, 159-167.

• S. Campello, R.A. Lacalle, M. Bettella, S. Manes, L. Scorrano and A. Viola; (2006) “Orchestration of lymphocyte chemotaxis by mitochondrial dynamics.” JEM 203, 2879-2886.

• S. Campello*, U. De Marchi*, I. Szabò, F. Tombola, J.-C. Martinou and M. Zoratti; (2005) “The properties of the mitochondrial megachannel in mitoplasts from human colon carcinoma cells are not influenced by Bax.” FEBS Letters 579, 3695-3700. [*both the authors contributed equally]

• U. De Marchi*, S. Campello*, I. Szabò, F. Tombola, J.-C. Martinou and M. Zoratti; ( 2004) “Bax does not directly participate in the Ca²⁺-induced permeability transition of isolated mitochondria.” J Biol Chem. 279, 37415-37422. [* both the authors contributed equally]

• F. Tombola, S. Campello, L. De Luca, P. Ruggiero, G. Del Giudice, E. Papini, M. Zoratti; (2003) “Plant polyphenols inhibit VacA, a toxin secreted by the gastric pathogen H. pylori” FEBS Letters 543, 184-189.

• S. Campello, F. Tombola, G. Cabrini and M. Zoratti; (2002) “The vacuolating toxin of Helicobacter pylori mimicks the CFTR-mediated chloride conductance.” FEBS Letters 532, 237-240.

• L. Morbiato, F. Tombola, S. Campello, G. Del Giudice, R. Rappuoli, M. Zoratti, E. Papini; (2001) “Vacuolation induced by VacA toxin of Helicobacter pylori requires the intracellular accumulation of membrane permeant bases, Cl^– and water.” FEBS Letters 508, 479-483.

• F. Tombola, C. Pagliaccia, S. Campello, J.L. Telford, C. Montecucco, E. Papini and M. Zoratti; (2001) “How the Loop and Middle Regions Influence the Properties of Helicobacter pylori VacA Channels.” Biophys. J. 81, 3204-3215.