The interest of our group is to investigate how signaling networks control key biological processes in physiological and pathological conditions.
Our main interest is focused on Acute Myeloid Leukemia (AML). Thanks to the AIRC grant, we are currently focusing on the mechanisms driving drug resistance in FLT3-dependent AML.
We apply a multidisciplinary strategy, where we combine high-throughput approaches, including mass spectrometry-based proteomics, phosphoproteomics and affinity proteomics, as well as transcriptomics with bioinformatic strategies to obtain predictive models of cancer cells.
We also interested to elucidate the connection between signaling pathways and metabolism control.
Reggio A, Rosina M, Krahmer N, Palma A, Petrilli LL, Maiolatesi G, Massacci G, Salvatori I, Valle C, Testa S, Gargioli C, Fuoco C, Castagnoli L, Cesareni G, Sacco F*. Metabolic reprogramming of fibro/adipogenic progenitors facilitates muscle regeneration. Life Sci Alliance. 2020 Feb 4;3(3).
Itzhak DN, Sacco F, Nagaraj N, Tyanova S, Mann M, Murgia M. SILAC-based quantitative proteomics using mass spectrometry quantifies endoplasmic reticulum stress in whole HeLa cells. Dis Model Mech. 2019 Nov 11;12(11)
Sacco F*, Seelig A, Humphrey SJ, Krahmer N, Volta F, Reggio A, Marchetti P, Gerdes J, Mann M. (2019). Phosphoproteomics Reveals the GSK3-PDX1 Axis as a Key Pathogenic Signaling Node in Diabetic Islets. Cell Metab. 2019 Jun 4;29(6):1422-1432.
Sacco, F., Perfetto, L. and Cesareni G. (2017) Combining phosphoproteomics datasets and literature information to reveal the functional connections in a cell phosphorylation network. Proteomics, 2018 18(5-6)..
Sacco, F., Caldarone, A., Castagnoli, L. and Cesareni G. (2016) The cell autonomous mechanisms underlying the activity of metformin as an anticancer drug. British Journal of Cancer, (12):1451-1456.
Sacco, F., Humphrey S., Cox, J., Mischnik, M., Klabunde, T., Schafer, M. and Mann, M. (2016) Glucose-regulated and drug perturbed beta-cell phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nature Communications, 7, 13250.
Sacco, F., Silvestri, A., Posca, D., Pirrò, S., Gherardini, Pier F., Castagnoli, L., Mann, M., and Cesareni, G. (2016) Deep Proteomics of Breast Cancer Cells Reveals that Metformin Rewires Signaling Networks Away from a Pro-growth State. Cell Systems 2, 2, 1-13.
Sacco F, Mattioni A, Boldt K, Panni S, Santonico E, Castagnoli L, Ueffing M, Cesareni G. A subset of RAB proteins modulates PP2A phosphatase activity. Sci Rep. 2016 Sep 9;6:32857. doi: 10.1038/srep32857.
Mischnik, M., Sacco, F., Cox, J., Schneider, H.C., Schafer, M., Hendlich, M., Crowther, D., Mann, M., and Klabunde, T. (2016). IKAP: A heuristic framework for inference of kinase activities from Phosphoproteomics data. Bioinformatics 32, 424-431.